Looks like the only requirement is Vcenter and Vsphere 4 client. So version 4.0 and up is supported.
http://www.cisco.com/en/US/docs/app_ntwk_services/data_center_app_services/application_networking_manager/5.1/device_support/table/sdt.html
ANM 5.2 and 5.1 includes a plug-in that allows you to integrate ANM into a VMware virtual data center environment. VMware is a third-party product for creating and managing virtual data centers. Using VMware vSphere Client, you can access ANM functionality and manage the ACE real servers that provide load-balancing services for the virtual machines in your virtual data center.
To use the ANM 5.2/5.1 plug-in, you must use VMware vCenter Server and VMware vSphere Client 4.
For more information about using ANM in a VMware virtual data center environment, see the User Guide for the Cisco Application Networking Manager 5.2/5.1.