Hello,
By itself, enabling VPC feature and configuring it on 2 switches will not impact any existing traffic.
But assuming that your 2 switches are already interconnected in traditional STP design with some Vlans, moving this interco into VPC Peer-Link with the production Vlans into it will potentially impact these Vlans while you perform the change.
By the way, no need to reboot them.
Remi Astruc