1. Can I confirm that this is still the case? That thread was 3 years ago, but I suspect it's still valid given that if the ASA is in routing mode it's acting solely at L3 and therefore doesn't deal with MACs.
2. So if we added a separate router and put the firewall in transparent mode, this could be done, right?
I'm not sure about filtering MAC addresses but I believe that you can expose that service doing static NAT to the Public IP and then filter the access to it by putting an ACL in place (like "outside_access_in") and filter which networks you allow to reach that Public IP (assuming you have more than one public IP).
We don't know what networks we want to allow though; that's the problem. We want our staff from their phones and their laptops to be able to access this service from wherever they are in the world, without having to open up the VPN first. So their IPs could be anything.
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