No, you can only have one server certificate on the expressways, you'll need to generate a new CSR from EXP-E or your own from openssl (or any other tool you like), have it signed, and upload it.
If I recall correctly, it does call for a reboot so services can start using the new certificate.
If you keep the trust chain and the same CN/SAN there should be no problem with your traversal zone.
HTH
java
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