Good to know that.. Thank u for update.

I hope you have forward and reverse entries of ISE node in DNS server.Can you do nslookup to IP and ISE hostname and share the output here.

Hi Francesco,I have similar problem and my AD and ISE are on the same network and there no firewall rule created.and this is the output of command executed on isenslookup _ldap._tcp.dc._msdcs.(Mydomain)1 querytype srvTrying "_ldap._tcp.dc._msdcs.Mydo...