Yes technically you can do that, I have not done Nutanix migration first hand myself but here are some general guidelines.
Take certificate backups as well with private key, so that you can install in new setup.
for licensing you will have to generate a new token from your smart licensing portal and reregister, you will also most likely will have to join nodes to AD so keep your admin account handy.
for service interruption, it depends on how’s your deployment, for distributed deployment, you may want to start with admin nodes first, like migrate secondary admin, secondary MNT, one policy node .. do your tests. Then migrate primary MnT, primary admin and rest of PSN one at a time for minimum service disruption.
keep the existing VM shutdown few weeks until you have everything running smooth on new platform, don’t delete them immediately.
also don’t skip the cisco link I sent earlier, there is some specific requirements for Nutanix.
-hope this helps-
