Please let us see the configuration of the Switchport (show run int TYPE X/X)
1) To find out if the Switch is receiving the request from the Vista Supplicant try enabling these debugs:
debug aaa authentication
debug aaa authorization
debug dot1x all
2) To find out if the Switch if relaying the request back to the ACS Server, please enable this debug:
debug radius
Also see the status of the port while testing:
show dot1x interface TYPE X/X details [show dot1x interface fa0/3 details]
Do you receive any type of authentication requests in the ACS Server before unplug/plug ethernet cable?
HTH,