Rizwan
The packet capture shows a few different 192.168.0.x IPs talking to the database server.
Are these also web servers and if so do they have any problems ?
In addition is this a new application or new web server ie. is the server successfully communicating with the database server for any other applications ?
And if the other IPs are web servers can you run the same application from them or it is only on just this one web server ?
If there are other web servers and they can run the same application then what would be really helpful is to refine your packet capture ie.
1) run a capture specifically for a web server that works
2) run a capture for the web server that doesn't
I did have a quick look at the packet capture and I can't see anything obviously wrong although it's been a while since I looked at these so others may spot something I am missing.
You won't necessarily see the initial connection flags as your capture may have been run while those connections had already been setup.
Jon