Hi,
it will not at all affect any thing in the live enviroment, this commands enables the ports less than 3 seconds. make sure that ur not entering this command on the port which is getting uplinked to the other switch, bcoz that might form a temporary loop, so juz enable "spanning-tree portfast" only to the end host(eg. where the PC's N/w Printers are connected)
hope this helps.
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