Mail ERROR after ISE and SMTP setup

[CCC3]

I set smtp on window server 2012 r2 to receive ISE's warning alarm by mail and succeeded from ise to that server and test connection

And
I set up my email in Configuration Changed, but it didn't come, and I checked it through tcp dump

I think I sent it
I checked the phrase "unable to relay for <e-mail>".

Does anyone know where I set it up wrong?

[balaji.bandi]

I set up my email in Configuration Changed, but it didn't come, and I checked it through tcp dump

this part was not clear - can you provide example ?