Brent,
This is more likely a configuration issue than anything else. Check/confirm the following:
1. Have the vlans been created on the 1000v and all upstream switches? "show vlan"
2. Are you allowing the VLAN on the trunk uplinks on each switch? "show int trunk"
Trace this through and you'll probably find it missing somewhere. If not, let us know and we'll go deeper. Also explain your topology including any device between the ping source and target.
Regards,
Robert